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TRANSVIHMI
nfernandez
Reads_Quality_Control
Commits
e7e37790
Commit
e7e37790
authored
3 years ago
by
Nicolas FERNANDEZ NUÑEZ
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Change Sickle-trim option for one letter than --word because issue segmentation fault 11
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README.md
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README.md
workflow/rules/reads_quality_control_pipeline.smk
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workflow/rules/reads_quality_control_pipeline.smk
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README.md
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e7e37790
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@@ -13,7 +13,7 @@ RQCP check NGS (illumina) reads quality and clean it if needed, as you set, usin
## Badges ##

,Catalina (10.15),Big Sure (11)/cyan?icon=apple&label&list=|&scale=0.9
>
)





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workflow/rules/reads_quality_control_pipeline.smk
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e7e37790
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@@ -194,18 +194,18 @@ rule sickletrim:
log:
"results/reports/sickle-trim/{sample}.log"
shell:
"sickle "
# Sickle, a windowed adaptive trimming tool for FASTQ files using quality
"{params.command} "
# Paired-end or single-end sequence trimming
"-
-qual-type
{params.encoding} "
# -t
: Type of quality values, solexa ; illumina ; sanger ; CASAVA, < 1.3 ; 1.3 to 1.7 ; >= 1.8
"-
-qual-threshold
{params.quality} " # -q: Threshold for trimming based on average quality in a window (default: 20)
"-
-length-treshold
{params.length} " # -
l
: Threshold to keep a read based on length after trimming (default: 20)
"-
-pe-file1
{input.forward} "
# -f
: Input paired-end forward fastq file
"-
-pe-file2
{input.reverse} "
# -r
: Input paired-end reverse fastq file
"-
-gzip-output "
# -
g
: Output gzipped files
"-
-output-pe1
{output.forward} "
# -o: Output trimmed forward fastq file
"-
-output-pe2
{output.reverse} "
# -p
: Output trimmed reverse fastq file (must use -s option)
"-
-output-single
{output.single} " # -
s
: Output trimmed singles fastq file
"&> {log}"
# Add redirection for log
"sickle " # Sickle, a windowed adaptive trimming tool for FASTQ files using quality
"{params.command} " # Paired-end or single-end sequence trimming
"-
t
{params.encoding} "
# --qual-type
: Type of quality values, solexa ; illumina ; sanger ; CASAVA, < 1.3 ; 1.3 to 1.7 ; >= 1.8
"-
q
{params.quality} "
#
-
-q
ual-threshold
: Threshold for trimming based on average quality in a window (default: 20)
"-
l
{params.length} "
# -
-length-threshold
: Threshold to keep a read based on length after trimming (default: 20)
"-
f
{input.forward} "
# --pe-file1
: Input paired-end forward fastq file
"-
r
{input.reverse} "
# --pe-file2
: Input paired-end reverse fastq file
"-
g "
# -
-gzip-output
: Output gzipped files
"-
o
{output.forward} " #
-
-o
utput-pe1
: Output trimmed forward fastq file
"-
p
{output.reverse} "
# --output-pe2
: Output trimmed reverse fastq file (must use -s option)
"-
s
{output.single} " # -
-output-single
: Output trimmed singles fastq file
"&> {log}" # Add redirection for log
###############################################################################
rule cutadapt:
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