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TRANSVIHMI
nfernandez
Reads_Quality_Control
Commits
e47dca80
Commit
e47dca80
authored
3 years ago
by
Nicolas FERNANDEZ NUÑEZ
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Change Sickle-trim option for one letter than --word because issue segmentation fault 11
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0453d1bf
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workflow/rules/reads_quality_control_pipeline.smk
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15 additions, 15 deletions
workflow/rules/reads_quality_control_pipeline.smk
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workflow/rules/reads_quality_control_pipeline.smk
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e47dca80
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@@ -5,7 +5,7 @@
...
@@ -5,7 +5,7 @@
# Aim: Reads Quality Control
# Aim: Reads Quality Control
# Date: 2021.04.30
# Date: 2021.04.30
# Run: snakemake --use-conda -s reads_quality_control_pipeline.smk --cores
# Run: snakemake --use-conda -s reads_quality_control_pipeline.smk --cores
# Latest modification: 2021.10.0
4
# Latest modification: 2021.10.0
7
# Todo: ND
# Todo: ND
###############################################################################
###############################################################################
...
@@ -168,8 +168,8 @@ rule fastqjoin:
...
@@ -168,8 +168,8 @@ rule fastqjoin:
"-o {output.forward} " # for unique forward files
"-o {output.forward} " # for unique forward files
"-o {output.reverse} " # for unique reverse files
"-o {output.reverse} " # for unique reverse files
"-o {output.joined} " # for join files
"-o {output.joined} " # for join files
#
"-r {log} " # Verbose stitch length report
"-r {log} " # Verbose stitch length report
"&> {log}" # Add redirection for log
#
"&> {log}" # Add redirection for log
###############################################################################
###############################################################################
rule sickletrim:
rule sickletrim:
...
@@ -194,18 +194,18 @@ rule sickletrim:
...
@@ -194,18 +194,18 @@ rule sickletrim:
log:
log:
"results/reports/sickle-trim/{sample}.log"
"results/reports/sickle-trim/{sample}.log"
shell:
shell:
"sickle " # Sickle, a windowed adaptive trimming tool for FASTQ files using quality
"sickle "
# Sickle, a windowed adaptive trimming tool for FASTQ files using quality
"{params.command} " # Paired-end or single-end sequence trimming
"{params.command} "
# Paired-end or single-end sequence trimming
"-
t
{params.encoding} "
# --qual-type
: Type of quality values, solexa ; illumina ; sanger ; CASAVA, < 1.3 ; 1.3 to 1.7 ; >= 1.8
"-
-qual-type
{params.encoding} "
# -t
: Type of quality values, solexa ; illumina ; sanger ; CASAVA, < 1.3 ; 1.3 to 1.7 ; >= 1.8
"-
q
{params.quality} "
#
-
-q
ual-threshold
: Threshold for trimming based on average quality in a window (default: 20)
"-
-qual-threshold
{params.quality} " # -q: Threshold for trimming based on average quality in a window (default: 20)
"-
l
{params.length} "
#
-
-l
ength-treshold
: Threshold to keep a read based on length after trimming (default: 20)
"-
-length-treshold
{params.length} " # -l: Threshold to keep a read based on length after trimming (default: 20)
"-
f
{input.forward} "
# --pe-file1
: Input paired-end forward fastq file
"-
-pe-file1
{input.forward} "
# -f
: Input paired-end forward fastq file
"-
r
{input.reverse} "
# --pe-file2
: Input paired-end reverse fastq file
"-
-pe-file2
{input.reverse} "
# -r
: Input paired-end reverse fastq file
"-
g "
# -
-gzip-output
: Output gzipped files
"-
-gzip-output "
# -
g
: Output gzipped files
"-
o
{output.forward} " #
-
-o
utput-pe1
: Output trimmed forward fastq file
"-
-output-pe1
{output.forward} "
# -o: Output trimmed forward fastq file
"-
p
{output.reverse} "
# --output-pe2
: Output trimmed reverse fastq file (must use -s option)
"-
-output-pe2
{output.reverse} "
# -p
: Output trimmed reverse fastq file (must use -s option)
"-
s
{output.single} " # -
-output-single
: Output trimmed singles fastq file
"-
-output-single
{output.single} " # -
s
: Output trimmed singles fastq file
"&> {log}" # Add redirection for log
"&> {log}"
# Add redirection for log
###############################################################################
###############################################################################
rule cutadapt:
rule cutadapt:
...
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