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TRANSVIHMI
nfernandez
Reads_Quality_Control
Commits
c828a526
Commit
c828a526
authored
2 years ago
by
nicolas.fernandez_ird.fr
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Update Snakemake-base env. (like geVarLi)
parent
857e68f6
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workflow/snakefiles/quality_control.smk
+7
-7
7 additions, 7 deletions
workflow/snakefiles/quality_control.smk
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7 additions
and
7 deletions
workflow/
ru
les/quality_control.smk
→
workflow/
snakefi
les/quality_control.smk
+
7
−
7
View file @
c828a526
...
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@@ -4,13 +4,13 @@
# Affiliation ____________ IRD_U233_TransVIHMI
# Aim ____________________ Snakefile with quality control rules
# Date ___________________ 2021.09.28
# Latest modifications ___ 202
2.11.18
# Latest modifications ___ 202
3.03.01
# Run ____________________ snakemake -s quality_control.smk --use-conda
###############################################################################
###### CONFIGURATION ######
configfile: "config/config.yaml"
configfile: "config
uration
/config.yaml"
###############################################################################
###### FUNCTIONS ######
...
...
@@ -38,9 +38,9 @@ MULTIQC = config["conda"][OS]["multiqc"] # MultiQC
###############################################################################
###### PARAMETERS ######
CONFIG = config["fastq-screen"]["config"] # Fastq-screen --conf
MAPP
ER = config["fastq-screen"]["
mapp
er"] # Fastq-screen --aligner
SUBSET = config["fastq-screen"]["subset"] # Fastq-screen --subset
CONFIG = config["fastq-screen"]["config"]
# Fastq-screen --conf
ALIGN
ER = config["fastq-screen"]["
align
er"] # Fastq-screen --aligner
SUBSET = config["fastq-screen"]["subset"]
# Fastq-screen --subset
###############################################################################
...
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@@ -86,7 +86,7 @@ rule fastqscreen_contamination_checking:
cpus = CPUS
params:
config = CONFIG,
mapper = MAPP
ER,
aligner = ALIGN
ER,
subset = SUBSET
input:
fastq = "resources/reads/"
...
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@@ -99,7 +99,7 @@ rule fastqscreen_contamination_checking:
"-q " # --quiet: Only show log warning
"--threads {resources.cpus} " # --threads: Specifies across how many threads bowtie will be allowed to run
"--conf {params.config} " # path to configuration file
"--aligner {params.
mapp
er} "
# -a: choose aligner 'bowtie', 'bowtie2', 'bwa'
"--aligner {params.
align
er} " # -a: choose aligner 'bowtie', 'bowtie2', 'bwa'
"--subset {params.subset} " # Don't use the whole sequence file, but create a subset of specified size
"--outdir {output.fastqscreen} " # Output directory
"{input.fastq}/*.fastq.gz " # Input file.fastq
...
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