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Commit 8d328f7c authored by nicolas.fernandez_ird.fr's avatar nicolas.fernandez_ird.fr :shinto_shrine:
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Doc: update features and tools

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# Features
# Fonctionnalités et outils
## Reads quality control
- Fastq-Screen (_contamination check_)
- FastQC (_quality metrics_)
- MultiQC (_html reports_)
## Reads cleaning
- Cutadapt (_adapters trimming & amplicon primers 'hard-clipping'_)
- Sickle-trim (_quality trimming_)
## Reads mapping
- Index genomes
- BWA aligments (bowtie2 / minimap2 also available)
- Bamclipper (_amplicon primers 'soft-clipping'_)
- Visualization (_output bam and bed for IGV_)
- Genome coverage (_statistics reports_)
## Variants calling
- ivar (_filtering on qualities_)
## Consensus sequences (_ivar output fasta file_)
## Genomes classification
- Nextclade (_consensus quality and lineages reports_)
- Pangolin (_lineages reports_)
## Gestionnaire de pipeline
- Workflow ................................. Snakemake
- Environnements ........................... Miniforge _(Conda)_
## Qualité contrôle des lectures
- Contrôle des contaminations .............. Fastq-Screen
- Mesures qualités des lectures ............ FastQC
- Rapport qualité HTML ..................... MultiQC
## Nettoyage des lectures
- Nettoyage des adaptateurs Illumina ....... Cutadapt
- Nettoyage des primers d'amplicons ........ Cutadapt _('hard-clipping')_
- Nettoyage des lectures basses qualités ... Sickle-trim
## Allignement des lectures
- Indexation des genomes ................... BWA _(ou minimap2, ou bowtie2)_
- Allignement des lectures ................. BWA _(ou minimap2, ou bowtie2)_
- Nettoyage des primers d'amplicons ........ Bamclipper _('soft-clipping')_
- Visualisation _(avec IGV)_ ................. fichiers BAM, BAI et BED
- Rapport de couverture génomique .......... Bedtools _(tableur)_
## Appel de variants
- Filtrage des variants ..................... Ivar
- Appel de variants ......................... Ivar
## Sequences consensus
- Appel du consensus ........................ Ivar _(fichier FASTA)_
## Classification génomiques
- Qualité des génomes SARS-CoV-2 ............ Nextclade
- Assignation lignées Nextclade ............. Nextclade
- Assignation lignées Pangolin .............. Nextclade _(ou Pangolin)_
## Rulegraph
<img src="../../../images/gevarli_rulegraph.png" width="1200" height="2400">
<img src="../../../images/gevarli_rulegraph.png" width="600" height="1200">
<img src="../../../images/gevarli_filegraph.png" width="300" height="1200">
## Filegraph
<img src="../../../images/gevarli_filegraph.png" width="1200" height="2400">
# Features
# Features and tools
## Workflow management
- Workflow ......................... Snakemake
- Environments ..................... Miniforge _(Conda)_
## Reads quality control
- Fastq-Screen (_contamination check_)
- FastQC (_quality metrics_)
- MultiQC (_html reports_)
- Contamination checking ........... Fastq-Screen
- Reads qualities metrics .......... FastQC
- HTML reporting ................... MultiQC
## Reads cleaning
- Cutadapt (_adapters trimming & amplicon primers 'hard-clipping'_)
- Sickle-trim (_quality trimming_)
- Illumina adpters trimming ........ Cutadapt
- Amplicon primers trimming ........ Cutadapt _('hard-clipping')_
- Reads quality trimming ........... Sickle-trim
## Reads mapping
- Index genomes
- BWA aligments (bowtie2 / minimap2 also available)
- Bamclipper (_amplicon primers 'soft-clipping'_)
- Visualization (_output bam and bed for IGV_)
- Genome coverage (_statistics reports_)
- Indexing genomes ................. BWA _(or minimap2, or bowtie2)_
- Reads mapping .................... BWA _(or minimap2, or bowtie2)_
- Amplicon primers trimming ........ Bamclipper _('soft-clipping')_
- Visualization _(with IGV)_ ......... BAM, BAI and BED files
- Genome coverage reports .......... Bedtools _(table file)_
## Variants calling
- ivar (_filtering on qualities_)
## Consensus sequences (_ivar output fasta file_)
- Variants filtering ............... Ivar
- Variants calling ................. Ivar
## Consensus sequences
- Consensus calling ................ Ivar _(FASTA file)_
## Genomes classification
- Nextclade (_consensus quality and lineages reports_)
- Pangolin (_lineages reports_)
- SARS-CoV-2 genome quality ........ Nextclade
- Nextclade lineages assignation ... Nextclade
- Pangolin lineages assignation .... Nextclade _(or Pangolin)_
## Rulegraph
<img src="../../../images/gevarli_rulegraph.png" width="1200" height="2400">
<img src="../../../images/gevarli_rulegraph.png" width="600" height="1200">
<img src="../../../images/gevarli_filegraph.png" width="300" height="1200">
## Filegraph
<img src="../../../images/gevarli_filegraph.png" width="1200" height="2400">
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