Skip to content
GitLab
Explore
Sign in
Primary navigation
Search or go to…
Project
GeVarLi
Manage
Activity
Members
Labels
Plan
Issues
Issue boards
Milestones
Code
Repository
Branches
Commits
Tags
Repository graph
Compare revisions
Snippets
Build
Pipelines
Jobs
Pipeline schedules
Artifacts
Help
Help
Support
GitLab documentation
Compare GitLab plans
Community forum
Contribute to GitLab
Provide feedback
Keyboard shortcuts
?
Snippets
Groups
Projects
Show more breadcrumbs
TRANSVIHMI
nfernandez
GeVarLi
Commits
8d328f7c
Commit
8d328f7c
authored
4 months ago
by
nicolas.fernandez_ird.fr
Browse files
Options
Downloads
Patches
Plain Diff
Doc: update features and tools
parent
61d41fbd
No related branches found
No related tags found
No related merge requests found
Pipeline
#45841
passed
4 months ago
Stage: deploy
Changes
2
Pipelines
1
Hide whitespace changes
Inline
Side-by-side
Showing
2 changed files
documentations/en/pages/features.md
+38
-23
38 additions, 23 deletions
documentations/en/pages/features.md
documentations/fr/pages/features.md
+33
-18
33 additions, 18 deletions
documentations/fr/pages/features.md
with
71 additions
and
41 deletions
documentations/en/pages/features.md
+
38
−
23
View file @
8d328f7c
# F
eature
s
# F
onctionnalités et outil
s
## Reads quality control
-
Fastq-Screen (_contamination check_)
-
FastQC (_quality metrics_)
-
MultiQC (_html reports_)
## Reads cleaning
-
Cutadapt (_adapters trimming & amplicon primers 'hard-clipping'_)
-
Sickle-trim (_quality trimming_)
## Reads mapping
-
Index genomes
-
BWA aligments (bowtie2 / minimap2 also available)
-
Bamclipper (_amplicon primers 'soft-clipping'_)
-
Visualization (_output bam and bed for IGV_)
-
Genome coverage (_statistics reports_)
## Variants calling
-
ivar (_filtering on qualities_)
## Consensus sequences (_ivar output fasta file_)
## Genomes classification
-
Nextclade (_consensus quality and lineages reports_)
-
Pangolin (_lineages reports_)
## Gestionnaire de pipeline
-
Workflow ................................. Snakemake
-
Environnements ........................... Miniforge _(Conda)_
## Qualité contrôle des lectures
-
Contrôle des contaminations .............. Fastq-Screen
-
Mesures qualités des lectures ............ FastQC
-
Rapport qualité HTML ..................... MultiQC
## Nettoyage des lectures
-
Nettoyage des adaptateurs Illumina ....... Cutadapt
-
Nettoyage des primers d'amplicons ........ Cutadapt _('hard-clipping')_
-
Nettoyage des lectures basses qualités ... Sickle-trim
## Allignement des lectures
-
Indexation des genomes ................... BWA _(ou minimap2, ou bowtie2)_
-
Allignement des lectures ................. BWA _(ou minimap2, ou bowtie2)_
-
Nettoyage des primers d'amplicons ........ Bamclipper _('soft-clipping')_
-
Visualisation _(avec IGV)_ ................. fichiers BAM, BAI et BED
-
Rapport de couverture génomique .......... Bedtools _(tableur)_
## Appel de variants
-
Filtrage des variants ..................... Ivar
-
Appel de variants ......................... Ivar
## Sequences consensus
-
Appel du consensus ........................ Ivar _(fichier FASTA)_
## Classification génomiques
-
Qualité des génomes SARS-CoV-2 ............ Nextclade
-
Assignation lignées Nextclade ............. Nextclade
-
Assignation lignées Pangolin .............. Nextclade _(ou Pangolin)_
## Rulegraph
<img
src=
"../../../images/gevarli_rulegraph.png"
width=
"1200"
height=
"2400"
>
<img
src=
"../../../images/gevarli_rulegraph.png"
width=
"600"
height=
"1200"
>
<img
src=
"../../../images/gevarli_filegraph.png"
width=
"300"
height=
"1200"
>
## Filegraph
<img
src=
"../../../images/gevarli_filegraph.png"
width=
"1200"
height=
"2400"
>
This diff is collapsed.
Click to expand it.
documentations/fr/pages/features.md
+
33
−
18
View file @
8d328f7c
# Features
# Features
and tools
## Workflow management
-
Workflow ......................... Snakemake
-
Environments ..................... Miniforge _(Conda)_
## Reads quality control
-
Fastq-Screen (_contamination check_)
-
FastQC (_quality metrics_)
-
MultiQC (_html reports_)
-
Contamination checking ........... Fastq-Screen
-
Reads qualities metrics .......... FastQC
-
HTML reporting ................... MultiQC
## Reads cleaning
-
Cutadapt (_adapters trimming & amplicon primers 'hard-clipping'_)
-
Sickle-trim (_quality trimming_)
-
Illumina adpters trimming ........ Cutadapt
-
Amplicon primers trimming ........ Cutadapt _('hard-clipping')_
-
Reads quality trimming ........... Sickle-trim
## Reads mapping
-
Index genomes
-
BWA aligments (bowtie2 / minimap2 also available)
-
Bamclipper (_amplicon primers 'soft-clipping'_)
-
Visualization (_output bam and bed for IGV_)
-
Genome coverage (_statistics reports_)
-
Indexing genomes ................. BWA _(or minimap2, or bowtie2)_
-
Reads mapping .................... BWA _(or minimap2, or bowtie2)_
-
Amplicon primers trimming ........ Bamclipper _('soft-clipping')_
-
Visualization _(with IGV)_ ......... BAM, BAI and BED files
-
Genome coverage reports .......... Bedtools _(table file)_
## Variants calling
-
ivar (_filtering on qualities_)
## Consensus sequences (_ivar output fasta file_)
-
Variants filtering ............... Ivar
-
Variants calling ................. Ivar
## Consensus sequences
-
Consensus calling ................ Ivar _(FASTA file)_
## Genomes classification
-
Nextclade (_consensus quality and lineages reports_)
-
Pangolin (_lineages reports_)
-
SARS-CoV-2 genome quality ........ Nextclade
-
Nextclade lineages assignation ... Nextclade
-
Pangolin lineages assignation .... Nextclade _(or Pangolin)_
## Rulegraph
<img
src=
"../../../images/gevarli_rulegraph.png"
width=
"1200"
height=
"2400"
>
<img
src=
"../../../images/gevarli_rulegraph.png"
width=
"600"
height=
"1200"
>
<img
src=
"../../../images/gevarli_filegraph.png"
width=
"300"
height=
"1200"
>
## Filegraph
<img
src=
"../../../images/gevarli_filegraph.png"
width=
"1200"
height=
"2400"
>
This diff is collapsed.
Click to expand it.
Preview
0%
Loading
Try again
or
attach a new file
.
Cancel
You are about to add
0
people
to the discussion. Proceed with caution.
Finish editing this message first!
Save comment
Cancel
Please
register
or
sign in
to comment